The Exploring of Pro- Inflammatory Cytokine IFN- γ, IL-17 and TNF- α in Inflammatory Cytokine  with Amoebic Dysentry from Kerbala Province

Sawsan Darweesh  Jari; Zahraa fadhil Abbas

doi:10.47577/eximia.v14i1.553

Authors

Sawsan Darweesh Jari Department of environmental health, College of Applied Medical Sciences, University of Kerbala, Karbala, Iraq
Zahraa fadhil Abbas Department of environmental health, College of Applied Medical Sciences, University of Kerbala, Karbala, Iraq

DOI:

https://doi.org/10.47577/eximia.v14i1.553

Keywords:

Inflammatory Cytokine, Amoebic Dysentry

Abstract

This research investigated the correlation between pro-inflammatory cytokines (IFN-γ, IL-17, and TNF-α) and Entamoeba histolytica infection in amoebic dysentery. The study examined the role of pro-inflammatory cytokines in preventing Entamoeba histolytica parasite infection. It measured IFN-γ, IL-17, and TNF-α concentrations in amoebiasis patients and compared them to healthy partisipants, demonstrating their importance. The study found a significant difference in IFN-γ, IL-17, and TNF-α concentrations in affected individuals (15.3 ± 1.5, 980.5 ± 23.4, and 29.4 ± 0.5 pg/ml) compared to the control group (6.5 ± 1.026, 658.6 ± 653, and 6.4 ± 0.9 pg/ml, respectively) (p ≤ p≤0.05). In addition, children ages 3-9 (78.5%) had the greatest infection rate, followed by 9–17 (71.4%), 24–30 (66.6%), and 17–24 (56.2%).. This research shows that amoebic dysentery patients had higher cytokine levels than healthy people. Age affects amoebic dysentery prevalence. Younger age groups, particularly children under 10, are more vulnerable to infection.

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References

1. Amoebic dysentery patients have much higher cytokine levels than healthy persons.

2. Age correlates with amoebic dysentery prevalence. According to research, children under 10 are more vulnerable to illness than older people.

References

Al-Ubaydi NA, Hadi ZS, Alkaniny ZA. Epidemiological and Immunological Study for Acute Amoebiasis Patients in Thi-Qar Governorate. J Int Pharm Res. 2019;46(5):555–60.

Atia AH (2009). Prevalence of intestinal parasites among children and old patients in Alexandria Nahia. J. Tech. 2009;22(2). 31427448; PMCID: PMC6803330.

Bansal D, Ave P, Kerneis S, Frileux P, Boché O, Baglin AC, et al. (2009). An ex-vivo human intestinal model to study Entamoeba histolytica pathogenesis. PLoS Negl Trop Dis. 2009 Nov;3(11). DOI: https://doi.org/10.1371/journal.pntd.0000551

Billet AC; Salmon Rousseau A; Piroth L; Martins C. (2019). An underestimated sexually transmitted infection: amoebiasis. BMJ Case Rep. 2019 May 10;12(5). DOI: https://doi.org/10.1136/bcr-2018-228942

Gordon S, Martinez FO. Alternative activation of macrophages: mechanism and functions. Immunity. 2010; 32:593–604. doi: 10.1016/j.immuni.2010.05.007. DOI: https://doi.org/10.1016/j.immuni.2010.05.007

Guillén , N. (2023). Pathogenicity and virulence of Entamoeba histolytica, the agent of amoebiasis .

Haque R, Huston CD, Hughes M, Houpt E, Petri WA. (2003). Amebiasis. N Engl J Med. 2003 Apr 17;348(16):1565-73. DOI: https://doi.org/10.1056/NEJMra022710

Jari , S.D., and Yousif, J.J. (2020). The effectiveness of silver nanoparticles loaded with albendazole and mebendazole drugs on the level of some immunological markers in male albino mice infected with hydatid cysts. Biochemical and Cellular Archives 20(2):4531-4538.

Kissoon-Singh V, Moreau F, Trusevych E, Chadee K. Entamoeba histolytica exacerbates epithelial tight junction permeability and proinflammatory responses in Muc2-/- mice. Am J Pathol. 2013 Mar;182(3):852–65. DOI: https://doi.org/10.1016/j.ajpath.2012.11.035

Lampel , K. A.; Al-Khaldi, S.; Cahill, S. M. (2012). Handbook of Foodborne Pathogenic Microorganisms and Natural Toxins. 2nd Edition . Chapter : Entamoeba histolytica. pp. 127.

Lidell ME, Moncada DM, Chadee K, HanssonC. (2006). Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel. Proceedings of the National Academy of Sciences 103 (24). 2006. DOI: https://doi.org/10.1073/pnas.0600623103

Mondal D, Minak J, Alam M, Liu Y, et al. (2012). Contribution of enteric infection, altered intestinal barrier function, and maternal malnutrition to infant malnutrition in Bangladesh. Clin Infect Dis. 2012;54(2):185–92. DOI: https://doi.org/10.1093/cid/cir807

Moonah SN, Abhyankar MM, Haque R, Petri WA. The Macrophage Migration Inhibitory Factor Homolog of Entamoeba histolytica Binds to and Immunomodulates Host Macrophages. Appleton JA, editor. Infect Immun. 2014 Sep;82(9):3523–30. DOI: https://doi.org/10.1128/IAI.01812-14

Morgan, G. A. ; Leech, N. A. ; Gloecner, G. W.; and Barrett, K. C. (2010). SPSS for Introductory Statistics: Use and Interpretation. 2nd ed. Lawrence Erlbaum Associates, publishers, Mahwah, New Jersey, London.

Noor Z, Watanabe K, Abhyankar MM, Burgess SL, Buonomo EL, Cowardin CA (2017). Role of eosinophils and tumor necrosis factor alpha in interleukin 25-mediated protection from amebic colitis. MBio. 2017;8(1). DOI: https://doi.org/10.1128/mBio.02329-16

Obaid HM (2014). The Effect of Entamoeba Histolytica and Giardia Lamblia Infection on Some Human Hematological Parameters. Vol. 4, Journal of Natural Sciences Research, www.iiste.org ISSN. Online; 2014.

Peterson K, Guo X, Elkahloun A, et al. The expression of REG 1A and REG 1B is increased during acute amebic colitis. Parasitol Int. 2011;60(3):296–300. DOI: https://doi.org/10.1016/j.parint.2011.04.005

Pulido-Ortega J, Talamás-Rohana P, Muñoz-Ortega MH, Aldaba-Muruato LR, Martínez Hernández SL, Campos-Esparza MDR, Cervantes-García D, Leon-Coria A, Moreau F, Chadee K, Ventura-Juárez. (2019). Functional Characterization of an Interferon Gamma Receptor-Like Protein on Entamoeba histolytica. Infect Immun. 2019 Oct 18;87(11): e00540-19. doi: 10.1128/IAI.00540-19. PMID: DOI: https://doi.org/10.1128/IAI.00540-19

Salit IE, Khairnar K, Gough K, Pillai DR. (2009). A possible cluster of sexually transmitted Entamoeba histolytica: genetic analysis of a highly virulent strain. Clin Infect Dis. 2009 Aug 01;49(3):346-53. DOI: https://doi.org/10.1086/600298

Stanley SL. (2003). Amoebiasis. Lancet. 2003 Mar 22;361(9362):1025-34. DOI: https://doi.org/10.1016/S0140-6736(03)12830-9

Uddin MJ, Leslie JL,ri WA. (2021). Host Protective Mechanisms to Intestinal Amebiasis. Trends Parasitol. 2021;37(2):165–75. Available from https://doi.org/10.1016/j.pt.2020.09.015. DOI: https://doi.org/10.1016/j.pt.2020.09.015

Virulence journal , Volume 14, 2023—Issue 1.

Zainab Abdul A, Al-quraishi. (2013). The Pattern of Leucocyte Parameters and C-Reactive Protein Findings of G. lamblia and E. histolytica Intestinal Infections in Children. Int J Recent Biotechnol. 2013;1(2):5–14.